ABSTRACT
@# Based on the community-based and family-centered concepts, the Chain Rehabilitation Services for children with cerebral palsy (CP) integrates all kinds of social resources to build the early screening network and community rehabilitation centers, and to enhance the integration of rehabilitation and educational services. This chain model makes it possible to coordinate the related institutes to provide better services for children with CP. Better services and better systematic management of children with CP in Shanghai and surrounding areas has also been achieved through this project.
ABSTRACT
OBJECTIVE: To establish a UPLC method for simultaneous determination of 5-hydroxymethyl furfural (1), leu-hetenone A (2), lectochrysin (3), and nootkatone (4) in Alpinia, oxyphylla Miq, and to compare the contents of the four components in different parts of this medicinal materials from different places. METHODS: The UPLC method was established on an HSS T3 Column (2.1 mm × 100 mm, 1.8 μm). The mobile phase consisted of water containing 0.1% formic acid and acetonilrile in gradient elution mode at the flow rate of 0.5 mL · min-1 and the detection wavelength was set at 255 nm. RESULTS: The standard curves of compounds 1, 2, 3, and 4 showed good linearity in the ranges of 1.223-24.46, 2.016-40.32, 1.875-37.50 and 16.78-335.6 μg · mL-1 with the corresponding average recoveries of 99.5%, 101.5%, 100.9% and 101.2%, respectively. CONCLUSION: The method is precise and highly reproducible, which can be used to simultaneously determine the antioxidant components including 5-hydroxymelhyl furfural, teuhetenone A, tectochrysin, and nootkatone in Alpinia oxyphylla Miq; compounds 2 and 4 are mainly extracted from the seeds, while compounds 1 and 3 come from the seeds and putamens equally. There are significant differences among the samples from different production areas.
ABSTRACT
The paper dealt with the characterization of polysaccharide of Paecilomyces lilacinus NH-PL-03 strain. First, we extracted and purified exude polysaccharide from the fungal fermentation broth by ethanol depositing method. Second, the proteins were removed by the Sevage method from the crude polysaccharide. Third, the purified polysaccharide (EP-1) was obtained after Superdex G-75 column separation. The results of UV-spectrometer and Sephacryl S-200 HR chromatography experiments showed that the EP-1 was a homogeneous pure polysaccharide with molecular weight of 35.2 kDa. Tested by paper chromatography analysis using the complete hydrolysis by sulfuric acid, we found that the EP-1 comprise single component as glucose. The chemical structure of EP-1 was confirmed as a kind of linear glucan linked by beta-(1,3) linkage. The Congo red reaction performed that EP-1 probable presented a triple-helical conformation in the dilute alkali.